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Image Search Results
Journal: Nature
Article Title: Morphodynamics of human early brain organoid development
doi: 10.1038/s41586-025-09151-3
Figure Lengend Snippet: a ) Photographs of the sample holding chamber with four separated sub-chambers each containing four microwells, for growing and imaging organoids with sixteen different organoids arranged one per microwell n = 16 organoids. b ) Schematic overview of tiled image acquisition and example images showing two timepoints, before and after tiled acquisition and image fusion. Scale Bar is 100 micrometers. Time is in hours. n = 16 organoids. c ) Cross section images (day 7) from 16 simultaneously imaged organoids, generated with cells lines labeled with nuclear membrane (lamin, RFP, magenta), plasma membrane (CAAX, RFP, magenta), actin (GFP, green), tubulin (RFP, magenta), and nuclei (histone, GFP, green) and unlabeled WTC-11. d ) Images showing cross section (z-plane) and orthogonal views (y-plane, x-plane) of one organoid from a timecourse lightsheet imaging experiment shown in c. Scale Bar is 100 micrometers. Time is in hours. e ) Maximum intensity projections from a 2-week imaging acquisition of a mosaic organoid (histone, GFP, green; CAAX, RFP, magenta), unlabeled WTC-11). Scale Bar is 500 micrometers. Time is in hours. n = 16 organoids. f ) Images from a 3-week continuous imaging experiment using a NKX2-1:GFP reporter line. The organoids were given SHH morphogen treatment to induce ventral telencephalic patterning of the organoids. Images are false-colored with the green-fire-blue LUT. Scale Bar is 100 micrometers. Time is in hours. n = 4 organoids.
Article Snippet: The organoids were treated with the average
Techniques: Imaging, Generated, Labeling, Membrane, Clinical Proteomics